IN VITRO FERTILIZATION
Fertilization of sperm and egg outside the body is called IVF
Ovarian follicles are stimulated to grow by gonadotrophins.
Several mature oocytes are aspirated from ovary by laparoscopy or by USG guided needle insertion
The oocytes are placed in a Petri dish containing a special culture medium and capacitated sperms
Fertilization of the oocytes and cleavage of the zygotes are monitored microscopically for 3 to 5 days
One to three of the resulting embryos (four- to eight-cell stage or early blastocysts) are transferred by introducing a catheter through the vagina and cervical canal into the uterus. Any remaining embryos are stored in liquid nitrogen for later use
Why use IVF?
1.Inadequate quality or quantity of sperm
2.Unhostile uterine environment for sperm
3.Sperm donation and oocyte donation
4.Surrogacy
