I can relate to that joy when being lazy make things work better. Guess I will give it a shot too for staining ki67.

Anyhow, few questions came to my mind when reading your post. Why did you need antigen retrieval for Ki67. Was it an antibody thing or was it because of fixation you used? Were these Oct samples fixed with PFA or were these paraffin embedded tissue sections?

Also am I looking at the normal non-cancerous colon section here? Is this the basal level of ki67 that we usually find in those glands there. They are glands right? It's kind of interesting.

Anyway, thanks for sharing this image and the post. Hope to read more from you.