The President insists he is "not a racist" despite reports of his derogatory comments about immigrants from Haiti and Africa.
Questioned by a reporter in Florida following reports that he said Haiti and Africa were "shithole countries" in a closed-door meeting, Mr Trump said: "No. I'm not a racist. I'm the least racist person you will ever interview."
The President also denied making the comments attributed to him, but didn't get into specifics about what he did or did not say.
"Did you see what various senators in the room said about my comments?" he said to the reporter. "They weren't made."
On Friday, Mr Trump tweeted a convoluted denial about his alleged choice of words, which triggered a global firestorm of criticism.
But Democratic Senator Dick Durbin, who attended the meeting between the lawmakers, said that "shithole" was the "exact word used by the President, not just once but repeatedly".